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Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion

In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone ind...

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Autores principales: Suchkov, Dmitry V., DeFlorio, Reagan, Draper, Edward, Ismael, Amber, Sukumar, Madhushalini, Arkowitz, Robert, Stone, David E.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869379/
https://www.ncbi.nlm.nih.gov/pubmed/20335504
http://dx.doi.org/10.1091/mbc.E09-08-0706
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author Suchkov, Dmitry V.
DeFlorio, Reagan
Draper, Edward
Ismael, Amber
Sukumar, Madhushalini
Arkowitz, Robert
Stone, David E.
author_facet Suchkov, Dmitry V.
DeFlorio, Reagan
Draper, Edward
Ismael, Amber
Sukumar, Madhushalini
Arkowitz, Robert
Stone, David E.
author_sort Suchkov, Dmitry V.
collection PubMed
description In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone induces its polarization into “crescents” that cap the future mating projection. Here, we find that in pheromone-treated cells, receptor crescents are visible before detectable polarization of actin cables and that the receptor can polarize in the absence of actin-dependent directed secretion. Receptor internalization, in contrast, seems to be essential for the generation of receptor polarity, and mutations that deregulate this process confer dramatic defects in directional sensing. We also show that pheromone induces the internalization and subsequent polarization of the mating-specific Gα and Gβ proteins and that the changes in G protein localization depend on receptor internalization and receptor–Gα coupling. Our data suggest that the polarization of the receptor and its G protein precedes actin polarization and is important for gradient sensing. We propose that the establishment of receptor/G protein polarity depends on a novel mechanism involving differential internalization and that this serves to amplify the shallow gradient of activated receptor across the cell.
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spelling pubmed-28693792010-07-30 Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion Suchkov, Dmitry V. DeFlorio, Reagan Draper, Edward Ismael, Amber Sukumar, Madhushalini Arkowitz, Robert Stone, David E. Mol Biol Cell Articles In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone induces its polarization into “crescents” that cap the future mating projection. Here, we find that in pheromone-treated cells, receptor crescents are visible before detectable polarization of actin cables and that the receptor can polarize in the absence of actin-dependent directed secretion. Receptor internalization, in contrast, seems to be essential for the generation of receptor polarity, and mutations that deregulate this process confer dramatic defects in directional sensing. We also show that pheromone induces the internalization and subsequent polarization of the mating-specific Gα and Gβ proteins and that the changes in G protein localization depend on receptor internalization and receptor–Gα coupling. Our data suggest that the polarization of the receptor and its G protein precedes actin polarization and is important for gradient sensing. We propose that the establishment of receptor/G protein polarity depends on a novel mechanism involving differential internalization and that this serves to amplify the shallow gradient of activated receptor across the cell. The American Society for Cell Biology 2010-05-15 /pmc/articles/PMC2869379/ /pubmed/20335504 http://dx.doi.org/10.1091/mbc.E09-08-0706 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Suchkov, Dmitry V.
DeFlorio, Reagan
Draper, Edward
Ismael, Amber
Sukumar, Madhushalini
Arkowitz, Robert
Stone, David E.
Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title_full Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title_fullStr Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title_full_unstemmed Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title_short Polarization of the Yeast Pheromone Receptor Requires Its Internalization but Not Actin-dependent Secretion
title_sort polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869379/
https://www.ncbi.nlm.nih.gov/pubmed/20335504
http://dx.doi.org/10.1091/mbc.E09-08-0706
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