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MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer

BACKGROUND: Advances in high-throughput technologies and bioinformatics have transformed gene expression profiling methodologies. The results of microarray experiments are often validated using reverse transcription quantitative PCR (RT-qPCR), which is the most sensitive and reproducible method to q...

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Detalles Bibliográficos
Autores principales: Chang, Kah Hoong, Mestdagh, Pieter, Vandesompele, Jo, Kerin, Michael J, Miller, Nicola
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873395/
https://www.ncbi.nlm.nih.gov/pubmed/20429937
http://dx.doi.org/10.1186/1471-2407-10-173