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MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
BACKGROUND: Advances in high-throughput technologies and bioinformatics have transformed gene expression profiling methodologies. The results of microarray experiments are often validated using reverse transcription quantitative PCR (RT-qPCR), which is the most sensitive and reproducible method to q...
Autores principales: | Chang, Kah Hoong, Mestdagh, Pieter, Vandesompele, Jo, Kerin, Michael J, Miller, Nicola |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873395/ https://www.ncbi.nlm.nih.gov/pubmed/20429937 http://dx.doi.org/10.1186/1471-2407-10-173 |
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