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Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
BACKGROUND: Trigonopsis variabilis D-amino acid oxidase (TvDAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operational stability and costs also vary with the field of application. Processes that...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873405/ https://www.ncbi.nlm.nih.gov/pubmed/20420682 http://dx.doi.org/10.1186/1475-2859-9-24 |