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Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification

BACKGROUND: PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. Fusion of PhaR-tagged self-cleavable Ssp DnaB intein to the N-terminus of a t...

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Detalles Bibliográficos
Autores principales: Zhang, Shuang, Wang, Zhi Hui, Chen, Guo Qiang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873406/
https://www.ncbi.nlm.nih.gov/pubmed/20459707
http://dx.doi.org/10.1186/1475-2859-9-28