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Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification
BACKGROUND: PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. Fusion of PhaR-tagged self-cleavable Ssp DnaB intein to the N-terminus of a t...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873406/ https://www.ncbi.nlm.nih.gov/pubmed/20459707 http://dx.doi.org/10.1186/1475-2859-9-28 |