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Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR
BACKGROUND: The detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA....
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873554/ https://www.ncbi.nlm.nih.gov/pubmed/20403208 http://dx.doi.org/10.1186/1471-2334-10-100 |