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Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

BACKGROUND: The detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA....

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Detalles Bibliográficos
Autores principales: Tomaso, Herbert, Kattar, Mireille, Eickhoff, Meike, Wernery, Ulrich, Al Dahouk, Sascha, Straube, Eberhard, Neubauer, Heinrich, Scholz, Holger C
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873554/
https://www.ncbi.nlm.nih.gov/pubmed/20403208
http://dx.doi.org/10.1186/1471-2334-10-100