Trypsin-induced proteome alteration during cell subculture in mammalian cells

BACKGROUND: It is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface proteins are often cleaved, which leads to dysregulati...

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Detalles Bibliográficos
Autores principales: Huang, Hsiang-Ling, Hsing, Hsiang-Wei, Lai, Tzu-Chia, Chen, Yi-Wen, Lee, Tian-Ren, Chan, Hsin-Tsu, Lyu, Ping-Chiang, Wu, Chieh-Lin, Lu, Ying-Chieh, Lin, Szu-Ting, Lin, Cheng-Wen, Lai, Chih-Ho, Chang, Hao-Teng, Chou, Hsiu-Chuan, Chan, Hong-Lin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873939/
https://www.ncbi.nlm.nih.gov/pubmed/20459778
http://dx.doi.org/10.1186/1423-0127-17-36

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873939/
https://www.ncbi.nlm.nih.gov/pubmed/20459778
http://dx.doi.org/10.1186/1423-0127-17-36

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