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Trypsin-induced proteome alteration during cell subculture in mammalian cells
BACKGROUND: It is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface proteins are often cleaved, which leads to dysregulati...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873939/ https://www.ncbi.nlm.nih.gov/pubmed/20459778 http://dx.doi.org/10.1186/1423-0127-17-36 |