A real-time assay for CpG-specific cytosine-C5 methyltransferase activity

A real-time assay for CpG-specific cytosine-C5 methyltransferase activity has been developed. The assay applies a break light oligonucleotide in which the methylation of an unmethylated 5′-CG-3′ site is enzymatically coupled to the development of a fluorescent signal. This sensitive assay can measur...

Descripción completa

Detalles Bibliográficos
Autores principales: Wood, Robert J., McKelvie, Jennifer C., Maynard-Smith, Michael D., Roach, Peter L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875032/
https://www.ncbi.nlm.nih.gov/pubmed/20139415
http://dx.doi.org/10.1093/nar/gkq047

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875032/
https://www.ncbi.nlm.nih.gov/pubmed/20139415
http://dx.doi.org/10.1093/nar/gkq047

Ejemplares similares