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A real-time assay for CpG-specific cytosine-C5 methyltransferase activity

A real-time assay for CpG-specific cytosine-C5 methyltransferase activity has been developed. The assay applies a break light oligonucleotide in which the methylation of an unmethylated 5′-CG-3′ site is enzymatically coupled to the development of a fluorescent signal. This sensitive assay can measur...

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Detalles Bibliográficos
Autores principales:	Wood, Robert J., McKelvie, Jennifer C., Maynard-Smith, Michael D., Roach, Peter L.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875032/ https://www.ncbi.nlm.nih.gov/pubmed/20139415 http://dx.doi.org/10.1093/nar/gkq047

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