Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes – A method for quantitative transcriptional reprogramming

Full-grown Xenopus oocytes in first meiotic prophase contain an immensely enlarged nucleus, the Germinal Vesicle (GV), that can be injected with several hundred somatic cell nuclei. When the nuclei of mammalian somatic cells or cultured cell lines are injected into a GV, a wide range of genes that a...

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Detalles Bibliográficos
Autores principales: Halley-Stott, R.P., Pasque, V., Astrand, C., Miyamoto, K., Simeoni, I., Jullien, J., Gurdon, J.B.
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877800/
https://www.ncbi.nlm.nih.gov/pubmed/20123126
http://dx.doi.org/10.1016/j.ymeth.2010.01.035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877800/
https://www.ncbi.nlm.nih.gov/pubmed/20123126
http://dx.doi.org/10.1016/j.ymeth.2010.01.035

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