Minimizing off-target signals in RNA fluorescent in situ hybridization

Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of ‘off-target’ hybridization is of crucial importance, but typical probes used fo...

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Detalles Bibliográficos
Autores principales: Arvey, Aaron, Hermann, Anita, Hsia, Cheryl C., Ie, Eugene, Freund, Yoav, McGinnis, William
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879521/
https://www.ncbi.nlm.nih.gov/pubmed/20164092
http://dx.doi.org/10.1093/nar/gkq042

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879521/
https://www.ncbi.nlm.nih.gov/pubmed/20164092
http://dx.doi.org/10.1093/nar/gkq042

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