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Systems Analysis of EGF Receptor Signaling Dynamics with Micro-Western Arrays

We describe micro-western arrays, which enable quantitative, sensitive and high-throughput assessment of protein abundance and modifications following electrophoretic separation of micro-arrayed cell lysates. This method allowed us to measure 91 phosphosites on 67 proteins at six time points followi...

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Detalles Bibliográficos
Autores principales: Ciaccio, Mark F., Wagner, Joel P., Chuu, Chih-Pin, Lauffenburger, Douglas A., Jones, Richard B.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881471/
https://www.ncbi.nlm.nih.gov/pubmed/20101245
http://dx.doi.org/10.1038/nmeth.1418