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Systems Analysis of EGF Receptor Signaling Dynamics with Micro-Western Arrays
We describe micro-western arrays, which enable quantitative, sensitive and high-throughput assessment of protein abundance and modifications following electrophoretic separation of micro-arrayed cell lysates. This method allowed us to measure 91 phosphosites on 67 proteins at six time points followi...
Autores principales: | Ciaccio, Mark F., Wagner, Joel P., Chuu, Chih-Pin, Lauffenburger, Douglas A., Jones, Richard B. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2881471/ https://www.ncbi.nlm.nih.gov/pubmed/20101245 http://dx.doi.org/10.1038/nmeth.1418 |
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