Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

BACKGROUND: Gene expression in lipopolysaccharide (LPS)-stimulated monocytes is mainly studied by quantitative real-time reverse transcription PCR (RT-qPCR) using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) or ACTB (beta-actin) as reference gene for normalization. Expression of traditional refe...

Descripción completa

Detalles Bibliográficos
Autores principales: Piehler, Armin P, Grimholt, Runa M, Øvstebø, Reidun, Berg, Jens P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884165/
https://www.ncbi.nlm.nih.gov/pubmed/20441576
http://dx.doi.org/10.1186/1471-2172-11-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884165/
https://www.ncbi.nlm.nih.gov/pubmed/20441576
http://dx.doi.org/10.1186/1471-2172-11-21

Ejemplares similares