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Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

BACKGROUND: Gene expression in lipopolysaccharide (LPS)-stimulated monocytes is mainly studied by quantitative real-time reverse transcription PCR (RT-qPCR) using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) or ACTB (beta-actin) as reference gene for normalization. Expression of traditional refe...

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Detalles Bibliográficos
Autores principales:	Piehler, Armin P, Grimholt, Runa M, Øvstebø, Reidun, Berg, Jens P
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Research article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884165/ https://www.ncbi.nlm.nih.gov/pubmed/20441576 http://dx.doi.org/10.1186/1471-2172-11-21

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