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G-stack modulated probe intensities on expression arrays - sequence corrections and signal calibration

BACKGROUND: The brightness of the probe spots on expression microarrays intends to measure the abundance of specific mRNA targets. Probes with runs of at least three guanines (G) in their sequence show abnormal high intensities which reflect rather probe effects than target concentrations. This G-bi...

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Detalles Bibliográficos
Autores principales: Fasold, Mario, Stadler, Peter F, Binder, Hans
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884167/
https://www.ncbi.nlm.nih.gov/pubmed/20423484
http://dx.doi.org/10.1186/1471-2105-11-207