Optimized design and data analysis of tag-based cytosine methylation assays

Using the type III restriction-modification enzyme EcoP15I, we isolated sequences flanking sites digested by the methylation-sensitive HpaII enzyme or its methylation-insensitive MspI isoschizomer for massively parallel sequencing. A novel data transformation allows us to normalise HpaII by MspI cou...

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Detalles Bibliográficos
Autores principales: Suzuki, Masako, Jing, Qiang, Lia, Daniel, Pascual, Marién, McLellan, Andrew, Greally, John M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884539/
https://www.ncbi.nlm.nih.gov/pubmed/20359321
http://dx.doi.org/10.1186/gb-2010-11-4-r36

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884539/
https://www.ncbi.nlm.nih.gov/pubmed/20359321
http://dx.doi.org/10.1186/gb-2010-11-4-r36

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