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Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist
Although the Enders strain of mumps virus (MuV) encodes a functional V protein that acts as an interferon (IFN) antagonist, in multi-cycle growth assays MuV Enders grew poorly in naïve (‘IFN-competent’ Hep2) cells but grew to high titres in ‘IFN-compromised’ Hep2 cells. Even so, the growth rate of M...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Society for General Microbiology
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885035/ https://www.ncbi.nlm.nih.gov/pubmed/19625458 http://dx.doi.org/10.1099/vir.0.013722-0 |
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author | Young, D. F. Galiano, M. C. Lemon, K. Chen, Y.-H. Andrejeva, J. Duprex, W. P. Rima, B. K. Randall, R. E. |
author_facet | Young, D. F. Galiano, M. C. Lemon, K. Chen, Y.-H. Andrejeva, J. Duprex, W. P. Rima, B. K. Randall, R. E. |
author_sort | Young, D. F. |
collection | PubMed |
description | Although the Enders strain of mumps virus (MuV) encodes a functional V protein that acts as an interferon (IFN) antagonist, in multi-cycle growth assays MuV Enders grew poorly in naïve (‘IFN-competent’ Hep2) cells but grew to high titres in ‘IFN-compromised’ Hep2 cells. Even so, the growth rate of MuV Enders was significantly slower in ‘IFN-compromised’ Hep2 cells when compared with its replication rate in Vero cells and with the replication rate of parainfluenza virus type 5 (a closely related paramyxovirus) in both naïve and ‘IFN-compromised’ Hep2 cells. This suggests that a consequence of slower growth is that the IFN system of naïve Hep2 cells can respond quickly enough to control the growth of MuV Enders. This is supported by the finding that rapidly growing variants of MuV Enders that were selected on ‘IFN-compromised’ Hep2 cells (i.e. in the absence of any selection pressure exerted by the IFN response) also grew to high titres on naïve Hep2 cells. Sequencing of the complete genome of one of these variants identified a single point mutation that resulted in a substitution of a conserved asparagine by histidine at position 498 of the haemagglutinin–neuraminidase protein, although this mutation was not present in all rapidly growing variants. These results support the concept that there is a race between the ability of a cell to detect and respond to virus infection and the ability of a virus to block the IFN response. Importantly, this emphasizes that factors other than viral IFN antagonists influence the sensitivity of viruses to IFN. |
format | Text |
id | pubmed-2885035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28850352010-07-06 Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist Young, D. F. Galiano, M. C. Lemon, K. Chen, Y.-H. Andrejeva, J. Duprex, W. P. Rima, B. K. Randall, R. E. J Gen Virol Animal Although the Enders strain of mumps virus (MuV) encodes a functional V protein that acts as an interferon (IFN) antagonist, in multi-cycle growth assays MuV Enders grew poorly in naïve (‘IFN-competent’ Hep2) cells but grew to high titres in ‘IFN-compromised’ Hep2 cells. Even so, the growth rate of MuV Enders was significantly slower in ‘IFN-compromised’ Hep2 cells when compared with its replication rate in Vero cells and with the replication rate of parainfluenza virus type 5 (a closely related paramyxovirus) in both naïve and ‘IFN-compromised’ Hep2 cells. This suggests that a consequence of slower growth is that the IFN system of naïve Hep2 cells can respond quickly enough to control the growth of MuV Enders. This is supported by the finding that rapidly growing variants of MuV Enders that were selected on ‘IFN-compromised’ Hep2 cells (i.e. in the absence of any selection pressure exerted by the IFN response) also grew to high titres on naïve Hep2 cells. Sequencing of the complete genome of one of these variants identified a single point mutation that resulted in a substitution of a conserved asparagine by histidine at position 498 of the haemagglutinin–neuraminidase protein, although this mutation was not present in all rapidly growing variants. These results support the concept that there is a race between the ability of a cell to detect and respond to virus infection and the ability of a virus to block the IFN response. Importantly, this emphasizes that factors other than viral IFN antagonists influence the sensitivity of viruses to IFN. Society for General Microbiology 2009-11 /pmc/articles/PMC2885035/ /pubmed/19625458 http://dx.doi.org/10.1099/vir.0.013722-0 Text en Copyright © 2009, SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Young, D. F. Galiano, M. C. Lemon, K. Chen, Y.-H. Andrejeva, J. Duprex, W. P. Rima, B. K. Randall, R. E. Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title | Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title_full | Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title_fullStr | Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title_full_unstemmed | Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title_short | Mumps virus Enders strain is sensitive to interferon (IFN) despite encoding a functional IFN antagonist |
title_sort | mumps virus enders strain is sensitive to interferon (ifn) despite encoding a functional ifn antagonist |
topic | Animal |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885035/ https://www.ncbi.nlm.nih.gov/pubmed/19625458 http://dx.doi.org/10.1099/vir.0.013722-0 |
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