Dilute-‘N’-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays

We have recently described a Dilute-‘N’-Go protocol that greatly simplifies preparation and sequencing of both strands of an amplicon generated using linear-after-the-exponential (LATE)-PCR, an advanced form of asymmetric PCR . The same protocol can also be used to sequence all limiting primer stran...

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Detalles Bibliográficos
Autores principales: Jia, Yanwei, Osborne, Adam, Rice, John E., Wangh, Lawrence J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887968/
https://www.ncbi.nlm.nih.gov/pubmed/20189962
http://dx.doi.org/10.1093/nar/gkq111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887968/
https://www.ncbi.nlm.nih.gov/pubmed/20189962
http://dx.doi.org/10.1093/nar/gkq111

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