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Dilute-‘N’-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays
We have recently described a Dilute-‘N’-Go protocol that greatly simplifies preparation and sequencing of both strands of an amplicon generated using linear-after-the-exponential (LATE)-PCR, an advanced form of asymmetric PCR . The same protocol can also be used to sequence all limiting primer stran...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887968/ https://www.ncbi.nlm.nih.gov/pubmed/20189962 http://dx.doi.org/10.1093/nar/gkq111 |