Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

BACKGROUND: We have developed an ultrasensitive method based on conventional PCR preamplification followed by nested amplification through real time PCR (qPCR) in the presence of the DNA intercalating agent EvaGreen. RESULTS: Amplification mixtures calibrated with a known number of pHV101 copies car...

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Detalles Bibliográficos
Autores principales: Hernández-Arteaga, Socorro, López-Revilla, Rubén
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888785/
https://www.ncbi.nlm.nih.gov/pubmed/20470388
http://dx.doi.org/10.1186/1750-9378-5-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888785/
https://www.ncbi.nlm.nih.gov/pubmed/20470388
http://dx.doi.org/10.1186/1750-9378-5-9

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