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Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

BACKGROUND: We have developed an ultrasensitive method based on conventional PCR preamplification followed by nested amplification through real time PCR (qPCR) in the presence of the DNA intercalating agent EvaGreen. RESULTS: Amplification mixtures calibrated with a known number of pHV101 copies car...

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Detalles Bibliográficos
Autores principales:	Hernández-Arteaga, Socorro, López-Revilla, Rubén
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888785/ https://www.ncbi.nlm.nih.gov/pubmed/20470388 http://dx.doi.org/10.1186/1750-9378-5-9

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