Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells

A previously engineered Methanocaldococcus jannaschii [Image: see text]–tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting [Image: see text]-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluo...

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Detalles Bibliográficos
Autores principales: Thibodeaux, Gabrielle Nina, Liang, Xiang, Moncivais, Kathryn, Umeda, Aiko, Singer, Oded, Alfonta, Lital, Zhang, Zhiwen Jonathan
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889833/
https://www.ncbi.nlm.nih.gov/pubmed/20582317
http://dx.doi.org/10.1371/journal.pone.0011263

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889833/
https://www.ncbi.nlm.nih.gov/pubmed/20582317
http://dx.doi.org/10.1371/journal.pone.0011263

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