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Localization of BDNF mRNA with the Huntington's disease protein in rat brain
BACKGROUND: Studies have implicated reduced levels of brain-derived neurotrophic factor (BDNF) in the pathogenesis of Huntington's disease. Mutant huntingtin (Htt) protein was previously reported to decrease BDNF gene transcription and axonal transport of BDNF. We recently showed that wild-type...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889995/ https://www.ncbi.nlm.nih.gov/pubmed/20507609 http://dx.doi.org/10.1186/1750-1326-5-22 |
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author | Ma, Bin Culver, Brady P Baj, Gabriele Tongiorgi, Enrico Chao, Moses V Tanese, Naoko |
author_facet | Ma, Bin Culver, Brady P Baj, Gabriele Tongiorgi, Enrico Chao, Moses V Tanese, Naoko |
author_sort | Ma, Bin |
collection | PubMed |
description | BACKGROUND: Studies have implicated reduced levels of brain-derived neurotrophic factor (BDNF) in the pathogenesis of Huntington's disease. Mutant huntingtin (Htt) protein was previously reported to decrease BDNF gene transcription and axonal transport of BDNF. We recently showed that wild-type Htt is associated with the Argonaute 2 microRNA-processing enzyme involved in gene silencing. In dendrites, Htt co-localizes with components of neuronal granules and mRNAs, indicating that it might play a role in post-transcriptional processing/transport of dendritic mRNAs. RESULTS: We conducted imaging experiments in cultured cortical neurons to demonstrate the co-localization of endogenous Htt and BDNF mRNA in fixed cells, and co-trafficking of BDNF 3'UTR mRNA with endogenous and fluorescently tagged Htt in live neurons. We used an enhanced technique that combines FISH and immunofluorescent staining to co-localize BDNF mRNA with Htt, Ago2, CPEB and dynein in thick vibratome sections of the rat cortex. CONCLUSIONS: In cultured neurons and sections of the rat cortex, we found BDNF mRNA associated with Htt and components of neuronal RNA granules, which are centers for regulating RNA transport and local translation. Htt may play a role in post-transcriptional transport/targeting of mRNA for BDNF, thus contributing to neurotrophic support and neuron survival. |
format | Text |
id | pubmed-2889995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28899952010-06-23 Localization of BDNF mRNA with the Huntington's disease protein in rat brain Ma, Bin Culver, Brady P Baj, Gabriele Tongiorgi, Enrico Chao, Moses V Tanese, Naoko Mol Neurodegener Research Article BACKGROUND: Studies have implicated reduced levels of brain-derived neurotrophic factor (BDNF) in the pathogenesis of Huntington's disease. Mutant huntingtin (Htt) protein was previously reported to decrease BDNF gene transcription and axonal transport of BDNF. We recently showed that wild-type Htt is associated with the Argonaute 2 microRNA-processing enzyme involved in gene silencing. In dendrites, Htt co-localizes with components of neuronal granules and mRNAs, indicating that it might play a role in post-transcriptional processing/transport of dendritic mRNAs. RESULTS: We conducted imaging experiments in cultured cortical neurons to demonstrate the co-localization of endogenous Htt and BDNF mRNA in fixed cells, and co-trafficking of BDNF 3'UTR mRNA with endogenous and fluorescently tagged Htt in live neurons. We used an enhanced technique that combines FISH and immunofluorescent staining to co-localize BDNF mRNA with Htt, Ago2, CPEB and dynein in thick vibratome sections of the rat cortex. CONCLUSIONS: In cultured neurons and sections of the rat cortex, we found BDNF mRNA associated with Htt and components of neuronal RNA granules, which are centers for regulating RNA transport and local translation. Htt may play a role in post-transcriptional transport/targeting of mRNA for BDNF, thus contributing to neurotrophic support and neuron survival. BioMed Central 2010-05-27 /pmc/articles/PMC2889995/ /pubmed/20507609 http://dx.doi.org/10.1186/1750-1326-5-22 Text en Copyright ©2010 Ma et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ma, Bin Culver, Brady P Baj, Gabriele Tongiorgi, Enrico Chao, Moses V Tanese, Naoko Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title | Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title_full | Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title_fullStr | Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title_full_unstemmed | Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title_short | Localization of BDNF mRNA with the Huntington's disease protein in rat brain |
title_sort | localization of bdnf mrna with the huntington's disease protein in rat brain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889995/ https://www.ncbi.nlm.nih.gov/pubmed/20507609 http://dx.doi.org/10.1186/1750-1326-5-22 |
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