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Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
BACKGROUND: Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form. Currently, in most cases the developmental line is characterised by a screening stage which is performed under batch co...
Autores principales: | Šiurkus, Juozas, Panula-Perälä, Johanna, Horn, Uwe, Kraft, Mario, Rimšeliene, Renata, Neubauer, Peter |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890543/ https://www.ncbi.nlm.nih.gov/pubmed/20487563 http://dx.doi.org/10.1186/1475-2859-9-35 |
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