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The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells
OBJECTIVES: To explore the function of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells, and discuss the signal transduetion mechanism of HepG2 cells in the apoptosis process induced by DADS by using the inhibitors of p38MAPK (SB203580) and caspase-3 (Z-DEVD-FMK). METHODS: After...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890544/ https://www.ncbi.nlm.nih.gov/pubmed/20478073 http://dx.doi.org/10.1186/1756-9966-29-50 |
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author | Ji, Chunxiao Ren, Fenglian Ma, Heng Xu, Ming |
author_facet | Ji, Chunxiao Ren, Fenglian Ma, Heng Xu, Ming |
author_sort | Ji, Chunxiao |
collection | PubMed |
description | OBJECTIVES: To explore the function of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells, and discuss the signal transduetion mechanism of HepG2 cells in the apoptosis process induced by DADS by using the inhibitors of p38MAPK (SB203580) and caspase-3 (Z-DEVD-FMK). METHODS: After the human HepG2 cells had been treated with the DADS and inhibitors for 24 h, cell viability was determined by the MTT method, apoptosis was evaluated by flow cytometry (FCM) and the expressions of p38MAPK and caspase-3 were measured by western-blot. RESULTS: Our results indicated that DADS activities the p38MAPK and caspase-3, but the inhibitors, SB203580 and Z-DEVD-FMK (for p38MAPKand for caspase-3, respectively), both have the effect of inhibitory activity on P38MAPK and caspase-3. Furthermore, a combination treatment with both DADS and inhibitor (SB203580 or Z-DEVD-FMK) decreases the inhibitory and apoptotic activity of HepG2 cells increased compared with DADS-treated. CONCLUSIONS: Our data indicate that p38MAPK and caspase-3 are involved in the process of DADS-induced apoptosis in human HepG2 cells and interact with each other. |
format | Text |
id | pubmed-2890544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28905442010-06-24 The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells Ji, Chunxiao Ren, Fenglian Ma, Heng Xu, Ming J Exp Clin Cancer Res Research OBJECTIVES: To explore the function of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells, and discuss the signal transduetion mechanism of HepG2 cells in the apoptosis process induced by DADS by using the inhibitors of p38MAPK (SB203580) and caspase-3 (Z-DEVD-FMK). METHODS: After the human HepG2 cells had been treated with the DADS and inhibitors for 24 h, cell viability was determined by the MTT method, apoptosis was evaluated by flow cytometry (FCM) and the expressions of p38MAPK and caspase-3 were measured by western-blot. RESULTS: Our results indicated that DADS activities the p38MAPK and caspase-3, but the inhibitors, SB203580 and Z-DEVD-FMK (for p38MAPKand for caspase-3, respectively), both have the effect of inhibitory activity on P38MAPK and caspase-3. Furthermore, a combination treatment with both DADS and inhibitor (SB203580 or Z-DEVD-FMK) decreases the inhibitory and apoptotic activity of HepG2 cells increased compared with DADS-treated. CONCLUSIONS: Our data indicate that p38MAPK and caspase-3 are involved in the process of DADS-induced apoptosis in human HepG2 cells and interact with each other. BioMed Central 2010-05-18 /pmc/articles/PMC2890544/ /pubmed/20478073 http://dx.doi.org/10.1186/1756-9966-29-50 Text en Copyright ©2010 Ji et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ji, Chunxiao Ren, Fenglian Ma, Heng Xu, Ming The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title | The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title_full | The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title_fullStr | The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title_full_unstemmed | The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title_short | The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells |
title_sort | roles of p38mapk and caspase-3 in dads-induced apoptosis in human hepg2 cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890544/ https://www.ncbi.nlm.nih.gov/pubmed/20478073 http://dx.doi.org/10.1186/1756-9966-29-50 |
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