Cargando…

UCE: A uracil excision (USER™)-based toolbox for transformation of cereals

BACKGROUND: Cloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or Agrobacterium-mediated transformation is hampered by a limited amount of unique restriction sites and by the difficulties often encountered when ligating small single strand DNA overhangs. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Hebelstrup, Kim H, Christiansen, Michael W, Carciofi, Massimiliano, Tauris, Birgitte, Brinch-Pedersen, Henrik, Holm, Preben B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892451/
https://www.ncbi.nlm.nih.gov/pubmed/20537147
http://dx.doi.org/10.1186/1746-4811-6-15