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UCE: A uracil excision (USER™)-based toolbox for transformation of cereals
BACKGROUND: Cloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or Agrobacterium-mediated transformation is hampered by a limited amount of unique restriction sites and by the difficulties often encountered when ligating small single strand DNA overhangs. The...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892451/ https://www.ncbi.nlm.nih.gov/pubmed/20537147 http://dx.doi.org/10.1186/1746-4811-6-15 |