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Expanding the ku70 toolbox for filamentous fungi: establishment of complementation vectors and recipient strains for advanced gene analyses

Mutants with a defective non-homologous-end-joining (NHEJ) pathway have boosted functional genomics in filamentous fungi as they are very efficient recipient strains for gene-targeting approaches, achieving homologous recombination frequencies up to 100%. For example, deletion of the ku70 homologous...

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Detalles Bibliográficos
Autores principales: Carvalho, Neuza D. S. P., Arentshorst, Mark, Jin Kwon, Min, Meyer, Vera, Ram, Arthur F. J.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892608/
https://www.ncbi.nlm.nih.gov/pubmed/20422182
http://dx.doi.org/10.1007/s00253-010-2588-1