Cargando…

Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment

Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an i...

Descripción completa

Detalles Bibliográficos
Autores principales: Mentlik, Ashley N., Sanborn, Keri B., Holzbaur, Erika L., Orange, Jordan S.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893988/
https://www.ncbi.nlm.nih.gov/pubmed/20444980
http://dx.doi.org/10.1091/mbc.E09-11-0930
_version_ 1782183111146078208
author Mentlik, Ashley N.
Sanborn, Keri B.
Holzbaur, Erika L.
Orange, Jordan S.
author_facet Mentlik, Ashley N.
Sanborn, Keri B.
Holzbaur, Erika L.
Orange, Jordan S.
author_sort Mentlik, Ashley N.
collection PubMed
description Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin reorganization preceding microtubule-organizing center (MTOC) polarization to the synapse. Because MTOC polarization to the synapse is required for polarization of lytic granules, we attempted to define their interrelationship. We found that compared with the time required for MTOC polarization, lytic granules converged to the MTOC rapidly. The MTOC-directed movement of lytic granules was independent of actin and microtubule reorganization, dependent on dynein motor function, occurred before MTOC polarization, and did not require a commitment to cytotoxicity. This defines a novel paradigm for rapid MTOC-directed transport as a prerequisite for directed secretion, one that may prepare, but not commit cells for precision secretory function.
format Text
id pubmed-2893988
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-28939882010-09-16 Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment Mentlik, Ashley N. Sanborn, Keri B. Holzbaur, Erika L. Orange, Jordan S. Mol Biol Cell Articles Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin reorganization preceding microtubule-organizing center (MTOC) polarization to the synapse. Because MTOC polarization to the synapse is required for polarization of lytic granules, we attempted to define their interrelationship. We found that compared with the time required for MTOC polarization, lytic granules converged to the MTOC rapidly. The MTOC-directed movement of lytic granules was independent of actin and microtubule reorganization, dependent on dynein motor function, occurred before MTOC polarization, and did not require a commitment to cytotoxicity. This defines a novel paradigm for rapid MTOC-directed transport as a prerequisite for directed secretion, one that may prepare, but not commit cells for precision secretory function. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893988/ /pubmed/20444980 http://dx.doi.org/10.1091/mbc.E09-11-0930 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Mentlik, Ashley N.
Sanborn, Keri B.
Holzbaur, Erika L.
Orange, Jordan S.
Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title_full Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title_fullStr Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title_full_unstemmed Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title_short Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
title_sort rapid lytic granule convergence to the mtoc in natural killer cells is dependent on dynein but not cytolytic commitment
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893988/
https://www.ncbi.nlm.nih.gov/pubmed/20444980
http://dx.doi.org/10.1091/mbc.E09-11-0930
work_keys_str_mv AT mentlikashleyn rapidlyticgranuleconvergencetothemtocinnaturalkillercellsisdependentondyneinbutnotcytolyticcommitment
AT sanbornkerib rapidlyticgranuleconvergencetothemtocinnaturalkillercellsisdependentondyneinbutnotcytolyticcommitment
AT holzbaurerikal rapidlyticgranuleconvergencetothemtocinnaturalkillercellsisdependentondyneinbutnotcytolyticcommitment
AT orangejordans rapidlyticgranuleconvergencetothemtocinnaturalkillercellsisdependentondyneinbutnotcytolyticcommitment