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Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment
Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an i...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893988/ https://www.ncbi.nlm.nih.gov/pubmed/20444980 http://dx.doi.org/10.1091/mbc.E09-11-0930 |
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author | Mentlik, Ashley N. Sanborn, Keri B. Holzbaur, Erika L. Orange, Jordan S. |
author_facet | Mentlik, Ashley N. Sanborn, Keri B. Holzbaur, Erika L. Orange, Jordan S. |
author_sort | Mentlik, Ashley N. |
collection | PubMed |
description | Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin reorganization preceding microtubule-organizing center (MTOC) polarization to the synapse. Because MTOC polarization to the synapse is required for polarization of lytic granules, we attempted to define their interrelationship. We found that compared with the time required for MTOC polarization, lytic granules converged to the MTOC rapidly. The MTOC-directed movement of lytic granules was independent of actin and microtubule reorganization, dependent on dynein motor function, occurred before MTOC polarization, and did not require a commitment to cytotoxicity. This defines a novel paradigm for rapid MTOC-directed transport as a prerequisite for directed secretion, one that may prepare, but not commit cells for precision secretory function. |
format | Text |
id | pubmed-2893988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28939882010-09-16 Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment Mentlik, Ashley N. Sanborn, Keri B. Holzbaur, Erika L. Orange, Jordan S. Mol Biol Cell Articles Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin reorganization preceding microtubule-organizing center (MTOC) polarization to the synapse. Because MTOC polarization to the synapse is required for polarization of lytic granules, we attempted to define their interrelationship. We found that compared with the time required for MTOC polarization, lytic granules converged to the MTOC rapidly. The MTOC-directed movement of lytic granules was independent of actin and microtubule reorganization, dependent on dynein motor function, occurred before MTOC polarization, and did not require a commitment to cytotoxicity. This defines a novel paradigm for rapid MTOC-directed transport as a prerequisite for directed secretion, one that may prepare, but not commit cells for precision secretory function. The American Society for Cell Biology 2010-07-01 /pmc/articles/PMC2893988/ /pubmed/20444980 http://dx.doi.org/10.1091/mbc.E09-11-0930 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Mentlik, Ashley N. Sanborn, Keri B. Holzbaur, Erika L. Orange, Jordan S. Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title | Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title_full | Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title_fullStr | Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title_full_unstemmed | Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title_short | Rapid Lytic Granule Convergence to the MTOC in Natural Killer Cells Is Dependent on Dynein But Not Cytolytic Commitment |
title_sort | rapid lytic granule convergence to the mtoc in natural killer cells is dependent on dynein but not cytolytic commitment |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893988/ https://www.ncbi.nlm.nih.gov/pubmed/20444980 http://dx.doi.org/10.1091/mbc.E09-11-0930 |
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