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The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex
All transport across the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs). Despite their enormous size, ∼60 MD in vertebrates, they are comprised of only ∼30 distinct proteins (nucleoporins or Nups), many of which form subcomplexes that act as building blocks for NPC assembly. One...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894445/ https://www.ncbi.nlm.nih.gov/pubmed/20566687 http://dx.doi.org/10.1083/jcb.200912045 |
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author | Theerthagiri, Gandhi Eisenhardt, Nathalie Schwarz, Heinz Antonin, Wolfram |
author_facet | Theerthagiri, Gandhi Eisenhardt, Nathalie Schwarz, Heinz Antonin, Wolfram |
author_sort | Theerthagiri, Gandhi |
collection | PubMed |
description | All transport across the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs). Despite their enormous size, ∼60 MD in vertebrates, they are comprised of only ∼30 distinct proteins (nucleoporins or Nups), many of which form subcomplexes that act as building blocks for NPC assembly. One of these evolutionarily conserved subcomplexes, the Nup93 complex, is a major structural component linking the NPC to the membranes of the NE. Using in vitro nuclear assembly assays, we show that two components of the Nup93 complex, Nup188 and Nup205, are dispensable for NPC formation. However, nuclei lacking Nup188 increase in size by several fold compared with wild type. We demonstrate that this phenotype is caused by an accelerated translocation of integral membrane proteins through NPCs, suggesting that Nup188 confines the passage of membrane proteins and is thus crucial for the homeostasis of the different nuclear membranes. |
format | Text |
id | pubmed-2894445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28944452010-12-28 The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex Theerthagiri, Gandhi Eisenhardt, Nathalie Schwarz, Heinz Antonin, Wolfram J Cell Biol Research Articles All transport across the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs). Despite their enormous size, ∼60 MD in vertebrates, they are comprised of only ∼30 distinct proteins (nucleoporins or Nups), many of which form subcomplexes that act as building blocks for NPC assembly. One of these evolutionarily conserved subcomplexes, the Nup93 complex, is a major structural component linking the NPC to the membranes of the NE. Using in vitro nuclear assembly assays, we show that two components of the Nup93 complex, Nup188 and Nup205, are dispensable for NPC formation. However, nuclei lacking Nup188 increase in size by several fold compared with wild type. We demonstrate that this phenotype is caused by an accelerated translocation of integral membrane proteins through NPCs, suggesting that Nup188 confines the passage of membrane proteins and is thus crucial for the homeostasis of the different nuclear membranes. The Rockefeller University Press 2010-06-28 /pmc/articles/PMC2894445/ /pubmed/20566687 http://dx.doi.org/10.1083/jcb.200912045 Text en © 2010 Theerthagiri et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Theerthagiri, Gandhi Eisenhardt, Nathalie Schwarz, Heinz Antonin, Wolfram The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title | The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title_full | The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title_fullStr | The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title_full_unstemmed | The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title_short | The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex |
title_sort | nucleoporin nup188 controls passage of membrane proteins across the nuclear pore complex |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2894445/ https://www.ncbi.nlm.nih.gov/pubmed/20566687 http://dx.doi.org/10.1083/jcb.200912045 |
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