Floxin, a resource for genetically engineering mouse embryonic stem cells

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations are reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is...

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Detalles Bibliográficos
Autores principales: Singla, Veena, Hunkapiller, Julie, Santos, Nicole, Seol, Allen, Norman, Andrew R., Wakenite, Paul, Skarnes, William C., Reiter, Jeremy F.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895430/
https://www.ncbi.nlm.nih.gov/pubmed/19966808
http://dx.doi.org/10.1038/nmeth.1406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895430/
https://www.ncbi.nlm.nih.gov/pubmed/19966808
http://dx.doi.org/10.1038/nmeth.1406

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