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Super-Resolution Video Microscopy of Live Cells by Structured Illumination

Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We...

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Detalles Bibliográficos
Autores principales: Kner, Peter, Chhun, Bryant B., Griffis, Eric R., Winoto, Lukman, Gustafsson, Mats G. L.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/
https://www.ncbi.nlm.nih.gov/pubmed/19404253
http://dx.doi.org/10.1038/nmeth.1324