Super-Resolution Video Microscopy of Live Cells by Structured Illumination

Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We...

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Detalles Bibliográficos
Autores principales: Kner, Peter, Chhun, Bryant B., Griffis, Eric R., Winoto, Lukman, Gustafsson, Mats G. L.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/
https://www.ncbi.nlm.nih.gov/pubmed/19404253
http://dx.doi.org/10.1038/nmeth.1324
Descripción
Sumario:Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila S2 cells in the total internal reflection (TIRF) mode.

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