Super-Resolution Video Microscopy of Live Cells by Structured Illumination

Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We...

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Detalles Bibliográficos
Autores principales: Kner, Peter, Chhun, Bryant B., Griffis, Eric R., Winoto, Lukman, Gustafsson, Mats G. L.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/
https://www.ncbi.nlm.nih.gov/pubmed/19404253
http://dx.doi.org/10.1038/nmeth.1324
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author Kner, Peter
Chhun, Bryant B.
Griffis, Eric R.
Winoto, Lukman
Gustafsson, Mats G. L.
author_facet Kner, Peter
Chhun, Bryant B.
Griffis, Eric R.
Winoto, Lukman
Gustafsson, Mats G. L.
author_sort Kner, Peter
collection PubMed
description Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila S2 cells in the total internal reflection (TIRF) mode.
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spelling pubmed-28955552010-07-01 Super-Resolution Video Microscopy of Live Cells by Structured Illumination Kner, Peter Chhun, Bryant B. Griffis, Eric R. Winoto, Lukman Gustafsson, Mats G. L. Nat Methods Article Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila S2 cells in the total internal reflection (TIRF) mode. 2009-04-26 2009-05 /pmc/articles/PMC2895555/ /pubmed/19404253 http://dx.doi.org/10.1038/nmeth.1324 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kner, Peter
Chhun, Bryant B.
Griffis, Eric R.
Winoto, Lukman
Gustafsson, Mats G. L.
Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title_full Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title_fullStr Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title_full_unstemmed Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title_short Super-Resolution Video Microscopy of Live Cells by Structured Illumination
title_sort super-resolution video microscopy of live cells by structured illumination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/
https://www.ncbi.nlm.nih.gov/pubmed/19404253
http://dx.doi.org/10.1038/nmeth.1324
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AT winotolukman superresolutionvideomicroscopyoflivecellsbystructuredillumination
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