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Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture
Recent evidence suggests that the timing of DNA replication is coordinated across megabase-scale domains in metazoan genomes, yet the importance of this aspect of genome organization is unclear. Here we show that replication timing is remarkably conserved between human and mouse, uncovering large re...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895651/ https://www.ncbi.nlm.nih.gov/pubmed/20617169 http://dx.doi.org/10.1371/journal.pgen.1001011 |
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author | Yaffe, Eitan Farkash-Amar, Shlomit Polten, Andreas Yakhini, Zohar Tanay, Amos Simon, Itamar |
author_facet | Yaffe, Eitan Farkash-Amar, Shlomit Polten, Andreas Yakhini, Zohar Tanay, Amos Simon, Itamar |
author_sort | Yaffe, Eitan |
collection | PubMed |
description | Recent evidence suggests that the timing of DNA replication is coordinated across megabase-scale domains in metazoan genomes, yet the importance of this aspect of genome organization is unclear. Here we show that replication timing is remarkably conserved between human and mouse, uncovering large regions that may have been governed by similar replication dynamics since these species have diverged. This conservation is both tissue-specific and independent of the genomic G+C content conservation. Moreover, we show that time of replication is globally conserved despite numerous large-scale genome rearrangements. We systematically identify rearrangement fusion points and demonstrate that replication time can be locally diverged at these loci. Conversely, rearrangements are shown to be correlated with early replication and physical chromosomal proximity. These results suggest that large chromosomal domains of coordinated replication are shuffled by evolution while conserving the large-scale nuclear architecture of the genome. |
format | Text |
id | pubmed-2895651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28956512010-07-08 Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture Yaffe, Eitan Farkash-Amar, Shlomit Polten, Andreas Yakhini, Zohar Tanay, Amos Simon, Itamar PLoS Genet Research Article Recent evidence suggests that the timing of DNA replication is coordinated across megabase-scale domains in metazoan genomes, yet the importance of this aspect of genome organization is unclear. Here we show that replication timing is remarkably conserved between human and mouse, uncovering large regions that may have been governed by similar replication dynamics since these species have diverged. This conservation is both tissue-specific and independent of the genomic G+C content conservation. Moreover, we show that time of replication is globally conserved despite numerous large-scale genome rearrangements. We systematically identify rearrangement fusion points and demonstrate that replication time can be locally diverged at these loci. Conversely, rearrangements are shown to be correlated with early replication and physical chromosomal proximity. These results suggest that large chromosomal domains of coordinated replication are shuffled by evolution while conserving the large-scale nuclear architecture of the genome. Public Library of Science 2010-07-01 /pmc/articles/PMC2895651/ /pubmed/20617169 http://dx.doi.org/10.1371/journal.pgen.1001011 Text en Yaffe et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yaffe, Eitan Farkash-Amar, Shlomit Polten, Andreas Yakhini, Zohar Tanay, Amos Simon, Itamar Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title | Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title_full | Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title_fullStr | Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title_full_unstemmed | Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title_short | Comparative Analysis of DNA Replication Timing Reveals Conserved Large-Scale Chromosomal Architecture |
title_sort | comparative analysis of dna replication timing reveals conserved large-scale chromosomal architecture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895651/ https://www.ncbi.nlm.nih.gov/pubmed/20617169 http://dx.doi.org/10.1371/journal.pgen.1001011 |
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