Cargando…

Directed evolution of recombinase specificity by split gene reassembly

The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities. In t...

Descripción completa

Detalles Bibliográficos
Autores principales: Gersbach, Charles A., Gaj, Thomas, Gordley, Russell M., Barbas, Carlos F.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896519/
https://www.ncbi.nlm.nih.gov/pubmed/20194120
http://dx.doi.org/10.1093/nar/gkq125