Transcripts expressed using a bicistronic vector pIREShyg2 are sensitized to nonsense-mediated mRNA decay

BACKGROUND: pIREShyg2 has been widely used as a bicistronic expression vector. However, it is not known if the vector would affect the expression of cloned genes via nonsense-mediated mRNA decay (NMD), an mRNA surveillance system that degrades mRNA with a premature termination codon (PTC). In mammal...

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Detalles Bibliográficos
Autores principales: Shikama, Yayoi, Hu, Huiyuan, Ohno, Makiko, Matsuoka, Isao, Shichishima, Tsutomu, Kimura, Junko
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896932/
https://www.ncbi.nlm.nih.gov/pubmed/20513249
http://dx.doi.org/10.1186/1471-2199-11-42

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896932/
https://www.ncbi.nlm.nih.gov/pubmed/20513249
http://dx.doi.org/10.1186/1471-2199-11-42

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