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Transcripts expressed using a bicistronic vector pIREShyg2 are sensitized to nonsense-mediated mRNA decay

BACKGROUND: pIREShyg2 has been widely used as a bicistronic expression vector. However, it is not known if the vector would affect the expression of cloned genes via nonsense-mediated mRNA decay (NMD), an mRNA surveillance system that degrades mRNA with a premature termination codon (PTC). In mammal...

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Detalles Bibliográficos
Autores principales:	Shikama, Yayoi, Hu, Huiyuan, Ohno, Makiko, Matsuoka, Isao, Shichishima, Tsutomu, Kimura, Junko
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896932/ https://www.ncbi.nlm.nih.gov/pubmed/20513249 http://dx.doi.org/10.1186/1471-2199-11-42

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