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Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation
Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, an...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Royal Society of Chemistry
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898651/ https://www.ncbi.nlm.nih.gov/pubmed/18931805 http://dx.doi.org/10.1039/b811501j |