Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors

BACKGROUND: The topoisomerase I (TopI) reaction intermediate consists of an enzyme covalently linked to a nicked DNA molecule, known as a TopI-DNA complex, that can be trapped by inhibitors and results in failure of re-ligation. Attempts at new derivative designs for TopI inhibition are enthusiastic...

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Detalles Bibliográficos
Autores principales: Tsai, Hsiang-Ping, Lin, Li-Wei, Lai, Zhi-Yang, Wu, Jui-Yu, Chen, Chiao-En, Hwang, Jaulang, Chen, Chien-Shu, Lin, Chun-Mao
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898767/
https://www.ncbi.nlm.nih.gov/pubmed/20565729
http://dx.doi.org/10.1186/1423-0127-17-49

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898767/
https://www.ncbi.nlm.nih.gov/pubmed/20565729
http://dx.doi.org/10.1186/1423-0127-17-49

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