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Purification of the CaaX-modified, dynamin-related large GTPase hGBP1 by coexpression with farnesyltransferase
Over a hundred proteins in eukaryotic cells carry a C-terminal CaaX box sequence, which targets them for posttranslational isoprenylation of the cysteine residue. This modification, catalyzed by either farnesyl or geranylgeranyl transferase, converts them into peripheral membrane proteins. Isoprenyl...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The American Society for Biochemistry and Molecular Biology
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2903825/ https://www.ncbi.nlm.nih.gov/pubmed/20348589 http://dx.doi.org/10.1194/jlr.D005397 |