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Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

BACKGROUND: A large amount of recombinant proteins can be synthesized in a few hours with Escherichia coli cell-free expression systems based on bacteriophage transcription. These cytoplasmic extracts are used in many applications that require large-scale protein production such as proteomics and hi...

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Detalles Bibliográficos
Autores principales: Shin, Jonghyeon, Noireaux, Vincent
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907309/
https://www.ncbi.nlm.nih.gov/pubmed/20594314
http://dx.doi.org/10.1186/1754-1611-4-9