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Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies
BACKGROUND: Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for mRNA quantification, but requires invariant expression of the chosen reference gene(s). In pathological myocardium, there is limited information on suitable reference genes other than the commonly used Gapdh mRNA an...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907514/ https://www.ncbi.nlm.nih.gov/pubmed/20331858 http://dx.doi.org/10.1186/1471-2199-11-22 |