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Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies

BACKGROUND: Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for mRNA quantification, but requires invariant expression of the chosen reference gene(s). In pathological myocardium, there is limited information on suitable reference genes other than the commonly used Gapdh mRNA an...

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Detalles Bibliográficos
Autores principales: Brattelid, Trond, Winer, Lisbeth H, Levy, Finn Olav, Liestøl, Knut, Sejersted, Ole M, Andersson, Kristin B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907514/
https://www.ncbi.nlm.nih.gov/pubmed/20331858
http://dx.doi.org/10.1186/1471-2199-11-22