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Eukaryotic gene invasion by a bacterial mobile insertion sequence element IS2 during cloning into a plasmid vector

Escherichia coli (E. coli) are commonly used as hosts for DNA cloning and sequencing. Upon transformation of E. coli with recombined vector carrying a gene of interest, the bacteria multiply the gene of interest while maintaining the integrity of its content. During the subcloning of a mouse genomic...

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Detalles Bibliográficos
Autores principales: Senejani, Alireza G, Sweasy, Joann B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907563/
https://www.ncbi.nlm.nih.gov/pubmed/20678256
http://dx.doi.org/10.1186/2041-9414-1-2