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Eukaryotic gene invasion by a bacterial mobile insertion sequence element IS2 during cloning into a plasmid vector
Escherichia coli (E. coli) are commonly used as hosts for DNA cloning and sequencing. Upon transformation of E. coli with recombined vector carrying a gene of interest, the bacteria multiply the gene of interest while maintaining the integrity of its content. During the subcloning of a mouse genomic...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907563/ https://www.ncbi.nlm.nih.gov/pubmed/20678256 http://dx.doi.org/10.1186/2041-9414-1-2 |