Real-Time Relative qPCR without Reference to Control Samples and Estimation of Run-Specific PCR Parameters from Run-Internal Mini-Standard Curves

BACKGROUND: Real-Time quantitative PCR is an important tool in research and clinical settings. Here, we describe two new approaches that broaden the scope of real-time quantitative PCR; namely, run-internal mini standard curves (RIMS) and direct real-time relative quantitative PCR (drqPCR). RIMS are...

Descripción completa

Detalles Bibliográficos
Autores principales: Bernth Jensen, Jens Magnus, Petersen, Mikkel Steen, Stegger, Marc, Østergaard, Lars J., Møller, Bjarne K.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908630/
https://www.ncbi.nlm.nih.gov/pubmed/20661435
http://dx.doi.org/10.1371/journal.pone.0011723

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908630/
https://www.ncbi.nlm.nih.gov/pubmed/20661435
http://dx.doi.org/10.1371/journal.pone.0011723

Ejemplares similares