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Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations

Site-specific labeling of Escherichia coli ribosomes has allowed application of single-molecule fluorescence spectroscopy and force methods to probe the mechanism of translation. To apply these approaches to eukaryotic translation, eukaryotic ribosomes must be specifically labeled with fluorescent l...

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Detalles Bibliográficos
Autores principales: Petrov, Alexey, Puglisi, Joseph D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910073/
https://www.ncbi.nlm.nih.gov/pubmed/20501598
http://dx.doi.org/10.1093/nar/gkq390