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Polyethyleneimine-mediated transfection of cultured postmitotic neurons from rat sympathetic ganglia and adult human retina

BACKGROUND: Chemical methods of transfection that have proven successful with cell lines often do not work with primary cultures of neurons. Recent data, however, suggest that linear polymers of the cation polyethyleneimine (PEI) can facilitate the uptake of nucleic acids by neurons. Consequently, w...

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Detalles Bibliográficos
Autores principales: Horbinski, Craig, Stachowiak, Michal K, Higgins, Dennis, Finnegan, Sarah G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC29102/
https://www.ncbi.nlm.nih.gov/pubmed/11231879
http://dx.doi.org/10.1186/1471-2202-2-2